anti-pstat1 tyr 701 Search Results


primary rabbit anti pstat1 tyr701  (Cell Signaling Technology Inc)
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anti pstat1  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc anti pstat1
Anti Pstat1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti pstat1 tyr701 rabbit polyclonal antibody  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc anti pstat1 tyr701 rabbit polyclonal antibody
Anti Pstat1 Tyr701 Rabbit Polyclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pstat1 tyr701 58d6 cell signaling technology ab 561284 cat  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc pstat1 tyr701 58d6 cell signaling technology ab 561284 cat
Pstat1 Tyr701 58d6 Cell Signaling Technology Ab 561284 Cat, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti pstat1 tyr701 mab  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc anti pstat1 tyr701 mab
Figure 2. SHP2 depletion upregulates <t>pSTAT1,</t> pJAK-1/2, and total STAT1. (A–F) PCI-13 and (G–L) SCC-90 cells were transfected with SHP2 siRNA (100 nmol/L, 48 hours), which significantly reduced SHP2 expression in HNC cells by Western blot analyses. SHP2 depletion also significantly upregulated pSTAT1 and its target gene product STAT1 as determined by intracellular flow cytometry and immunblotting compared with control siRNA in (B, C, E) PCI-13 and (H, I, K) SCC-90 cells. SHP2 depletion also upregulated pJAK1 (Tyr1022/1023) and pJAK2 (Tyr 1007/1008) compared with control siRNA in (F) PCI-13 and (L) SCC-90 cells. The cells were treated with IFN-g (100 U/mL ¼ 1,464 pg/mL, 48 hours) as a positive control for STAT1 phosphorylation. Data represent 3 independent experiments and error bars indicate SE.
Anti Pstat1 Tyr701 Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti pstat1 tyr701 mab/product/Cell Signaling Technology Inc
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anti pstat1 tyr701  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc anti pstat1 tyr701
Figure 2. SHP2 depletion upregulates <t>pSTAT1,</t> pJAK-1/2, and total STAT1. (A–F) PCI-13 and (G–L) SCC-90 cells were transfected with SHP2 siRNA (100 nmol/L, 48 hours), which significantly reduced SHP2 expression in HNC cells by Western blot analyses. SHP2 depletion also significantly upregulated pSTAT1 and its target gene product STAT1 as determined by intracellular flow cytometry and immunblotting compared with control siRNA in (B, C, E) PCI-13 and (H, I, K) SCC-90 cells. SHP2 depletion also upregulated pJAK1 (Tyr1022/1023) and pJAK2 (Tyr 1007/1008) compared with control siRNA in (F) PCI-13 and (L) SCC-90 cells. The cells were treated with IFN-g (100 U/mL ¼ 1,464 pg/mL, 48 hours) as a positive control for STAT1 phosphorylation. Data represent 3 independent experiments and error bars indicate SE.
Anti Pstat1 Tyr701, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti pstat1 tyr701/product/Cell Signaling Technology Inc
Average 98 stars, based on 1 article reviews
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anti pstat1  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology anti pstat1
Figure 2. SHP2 depletion upregulates <t>pSTAT1,</t> pJAK-1/2, and total STAT1. (A–F) PCI-13 and (G–L) SCC-90 cells were transfected with SHP2 siRNA (100 nmol/L, 48 hours), which significantly reduced SHP2 expression in HNC cells by Western blot analyses. SHP2 depletion also significantly upregulated pSTAT1 and its target gene product STAT1 as determined by intracellular flow cytometry and immunblotting compared with control siRNA in (B, C, E) PCI-13 and (H, I, K) SCC-90 cells. SHP2 depletion also upregulated pJAK1 (Tyr1022/1023) and pJAK2 (Tyr 1007/1008) compared with control siRNA in (F) PCI-13 and (L) SCC-90 cells. The cells were treated with IFN-g (100 U/mL ¼ 1,464 pg/mL, 48 hours) as a positive control for STAT1 phosphorylation. Data represent 3 independent experiments and error bars indicate SE.
Anti Pstat1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti phospho stat1  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc anti phospho stat1
Figure 2. SHP2 depletion upregulates <t>pSTAT1,</t> pJAK-1/2, and total STAT1. (A–F) PCI-13 and (G–L) SCC-90 cells were transfected with SHP2 siRNA (100 nmol/L, 48 hours), which significantly reduced SHP2 expression in HNC cells by Western blot analyses. SHP2 depletion also significantly upregulated pSTAT1 and its target gene product STAT1 as determined by intracellular flow cytometry and immunblotting compared with control siRNA in (B, C, E) PCI-13 and (H, I, K) SCC-90 cells. SHP2 depletion also upregulated pJAK1 (Tyr1022/1023) and pJAK2 (Tyr 1007/1008) compared with control siRNA in (F) PCI-13 and (L) SCC-90 cells. The cells were treated with IFN-g (100 U/mL ¼ 1,464 pg/mL, 48 hours) as a positive control for STAT1 phosphorylation. Data represent 3 independent experiments and error bars indicate SE.
Anti Phospho Stat1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pstat1 (tyr 701) antibody  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc pstat1 (tyr 701) antibody
Figure 2. SHP2 depletion upregulates <t>pSTAT1,</t> pJAK-1/2, and total STAT1. (A–F) PCI-13 and (G–L) SCC-90 cells were transfected with SHP2 siRNA (100 nmol/L, 48 hours), which significantly reduced SHP2 expression in HNC cells by Western blot analyses. SHP2 depletion also significantly upregulated pSTAT1 and its target gene product STAT1 as determined by intracellular flow cytometry and immunblotting compared with control siRNA in (B, C, E) PCI-13 and (H, I, K) SCC-90 cells. SHP2 depletion also upregulated pJAK1 (Tyr1022/1023) and pJAK2 (Tyr 1007/1008) compared with control siRNA in (F) PCI-13 and (L) SCC-90 cells. The cells were treated with IFN-g (100 U/mL ¼ 1,464 pg/mL, 48 hours) as a positive control for STAT1 phosphorylation. Data represent 3 independent experiments and error bars indicate SE.
Pstat1 (Tyr 701) Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti pstat1 tyr701 af647  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc anti pstat1 tyr701 af647
Figure 2. SHP2 depletion upregulates <t>pSTAT1,</t> pJAK-1/2, and total STAT1. (A–F) PCI-13 and (G–L) SCC-90 cells were transfected with SHP2 siRNA (100 nmol/L, 48 hours), which significantly reduced SHP2 expression in HNC cells by Western blot analyses. SHP2 depletion also significantly upregulated pSTAT1 and its target gene product STAT1 as determined by intracellular flow cytometry and immunblotting compared with control siRNA in (B, C, E) PCI-13 and (H, I, K) SCC-90 cells. SHP2 depletion also upregulated pJAK1 (Tyr1022/1023) and pJAK2 (Tyr 1007/1008) compared with control siRNA in (F) PCI-13 and (L) SCC-90 cells. The cells were treated with IFN-g (100 U/mL ¼ 1,464 pg/mL, 48 hours) as a positive control for STAT1 phosphorylation. Data represent 3 independent experiments and error bars indicate SE.
Anti Pstat1 Tyr701 Af647, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pe-conjugated anti-human pstat1-tyr-701 mab  (Becton Dickinson)
90
Becton Dickinson pe-conjugated anti-human pstat1-tyr-701 mab
Figure 2. SHP2 depletion upregulates <t>pSTAT1,</t> pJAK-1/2, and total STAT1. (A–F) PCI-13 and (G–L) SCC-90 cells were transfected with SHP2 siRNA (100 nmol/L, 48 hours), which significantly reduced SHP2 expression in HNC cells by Western blot analyses. SHP2 depletion also significantly upregulated pSTAT1 and its target gene product STAT1 as determined by intracellular flow cytometry and immunblotting compared with control siRNA in (B, C, E) PCI-13 and (H, I, K) SCC-90 cells. SHP2 depletion also upregulated pJAK1 (Tyr1022/1023) and pJAK2 (Tyr 1007/1008) compared with control siRNA in (F) PCI-13 and (L) SCC-90 cells. The cells were treated with IFN-g (100 U/mL ¼ 1,464 pg/mL, 48 hours) as a positive control for STAT1 phosphorylation. Data represent 3 independent experiments and error bars indicate SE.
Pe Conjugated Anti Human Pstat1 Tyr 701 Mab, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-human pstat1 (tyr 701)  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology anti-human pstat1 (tyr 701)
Figure 2. SHP2 depletion upregulates <t>pSTAT1,</t> pJAK-1/2, and total STAT1. (A–F) PCI-13 and (G–L) SCC-90 cells were transfected with SHP2 siRNA (100 nmol/L, 48 hours), which significantly reduced SHP2 expression in HNC cells by Western blot analyses. SHP2 depletion also significantly upregulated pSTAT1 and its target gene product STAT1 as determined by intracellular flow cytometry and immunblotting compared with control siRNA in (B, C, E) PCI-13 and (H, I, K) SCC-90 cells. SHP2 depletion also upregulated pJAK1 (Tyr1022/1023) and pJAK2 (Tyr 1007/1008) compared with control siRNA in (F) PCI-13 and (L) SCC-90 cells. The cells were treated with IFN-g (100 U/mL ¼ 1,464 pg/mL, 48 hours) as a positive control for STAT1 phosphorylation. Data represent 3 independent experiments and error bars indicate SE.
Anti Human Pstat1 (Tyr 701), supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 2. SHP2 depletion upregulates pSTAT1, pJAK-1/2, and total STAT1. (A–F) PCI-13 and (G–L) SCC-90 cells were transfected with SHP2 siRNA (100 nmol/L, 48 hours), which significantly reduced SHP2 expression in HNC cells by Western blot analyses. SHP2 depletion also significantly upregulated pSTAT1 and its target gene product STAT1 as determined by intracellular flow cytometry and immunblotting compared with control siRNA in (B, C, E) PCI-13 and (H, I, K) SCC-90 cells. SHP2 depletion also upregulated pJAK1 (Tyr1022/1023) and pJAK2 (Tyr 1007/1008) compared with control siRNA in (F) PCI-13 and (L) SCC-90 cells. The cells were treated with IFN-g (100 U/mL ¼ 1,464 pg/mL, 48 hours) as a positive control for STAT1 phosphorylation. Data represent 3 independent experiments and error bars indicate SE.

Journal: Clinical Cancer Research

Article Title: SHP2 Is Overexpressed and Inhibits pSTAT1-Mediated APM Component Expression, T-cell Attracting Chemokine Secretion, and CTL Recognition in Head and Neck Cancer Cells

doi: 10.1158/1078-0432.ccr-12-1517

Figure Lengend Snippet: Figure 2. SHP2 depletion upregulates pSTAT1, pJAK-1/2, and total STAT1. (A–F) PCI-13 and (G–L) SCC-90 cells were transfected with SHP2 siRNA (100 nmol/L, 48 hours), which significantly reduced SHP2 expression in HNC cells by Western blot analyses. SHP2 depletion also significantly upregulated pSTAT1 and its target gene product STAT1 as determined by intracellular flow cytometry and immunblotting compared with control siRNA in (B, C, E) PCI-13 and (H, I, K) SCC-90 cells. SHP2 depletion also upregulated pJAK1 (Tyr1022/1023) and pJAK2 (Tyr 1007/1008) compared with control siRNA in (F) PCI-13 and (L) SCC-90 cells. The cells were treated with IFN-g (100 U/mL ¼ 1,464 pg/mL, 48 hours) as a positive control for STAT1 phosphorylation. Data represent 3 independent experiments and error bars indicate SE.

Article Snippet: Anti-pSTAT1 Tyr701 mAb (Cell Signaling Tech), anti-total STAT1 (C-24) polyclonal (pAb; Santa Cruz Biotech), anti-b-actin mAb (Sigma-Aldrich Inc.), anti-rabbit IgG-HRP (Promega), anti-mouse IgG-HRP (Bio-Rad), anti-phosphorylated JAK-1 (Tyr1022/1023) mAb, antiphosphorylated JAK-2 (Tyr 1007/1008) mAb (Cell signaling Tech), anti-total JAK-1 mAb, and anti-total JAK-2 mAb (Santa Cruz Biotech) were used in immunoblot analyses.

Techniques: Transfection, Expressing, Western Blot, Cytometry, Control, Positive Control, Phospho-proteomics